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KMID : 0360220140550081208
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Journal of the Korean Ophthalmological Society
2014 Volume.55 No. 8 p.1208 ~ p.1212
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Effect of Bevacizumab and Ranibizumab on the Expression of eNOS in Trabecular Meshwork Cells
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Kim Se-Eun
Kim Jae-Woo
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Abstract
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Purpose: To compare the effects of bevacizumab and ranibizumab on the expression of eNOS in cultured human trabecular meshwork cells (HTMC).
Methods: Primarily cultured HTMC were exposed to 0.5 mg/mL bevacizumab and ranibizumab using 1% serum-containing media for 30 minutes. Expression of eNOS mRNA was assessed with RT-PCR. Additionally, after exposure to 20 ng/mL of vascular endothelial growth factor (VEGF) and 0.5 mg/mL bevacizumab and ranibizumab, the production of nitric oxide was assessed with Griess assay.
Results: VEGF increased the production of nitric oxide in HTMC. Bevacizumab and ranibizumab decreased the expression of eNOS mRNA and production of nitric oxide (p < 0.05) in HTMC. The decrease in eNOS mRNA expression and production of nitric oxide was not significant between bevacizumab and ranibizumab (p > 0.05).
Conclusions: In HTMC, both bevacizumab and ranibizumab decreased the expression of eNOS mRNA with no significant difference observed between the two drugs.
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KEYWORD
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Bevacizumab, eNOS, Nitric oxide, Ranibizumab, Trabecular meshwork
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